The District provides bus and light rail services, regional commuter and inter-city rail service, ADA paratransit service, congestion management, specific highway improvements and other transportation projects, and countywide transportation planning and funding.

The public can request, report, or receive the following services from the District: (1) Report stagnant water or report neglected pools; (2) Request free mosquitofish; (3) Request a rodent inspection; (4) Receive adult-mosquito-control treatment alerts; (5) Request a wildlife inspection; (6) Request a presentation or educational booth; (7) Report a dead bird; (8) Request a yellowjacket nest removal; (9) Request insect identification; (10) Subscribe to monthly newsletter; (11) Report a coyote/mountain lion sighting; and (12) Submit a public records request.

The District 's services include: 1) detection of the presence of vector-borne diseases such as West Nile Virus, rabies, Lyme Disease through ongoing and comprehensive vector surveillance and testing, 2) reducing vectors or exposure to vectors that transmit diseases, 3) conducting routine inspections and treatment as necessary of known mosquito and rodent sources, 4) responding to public service requests for identification, advice and/or initiation of control measures for mosquitoes, rodents, wildlife, and miscellaneous invertebrates (e.g., ticks, yellow jackets, cockroaches, bees, fleas, flies, etc.), 5) providing public information through a variety of channels, including a website, social media and educational presentations for schools, homeowners associations, private businesses, civic groups and other interested groups regarding vectors and vector-borne diseases, and 6) preventing disease and illness by identifying, inventorying, inspecting, and treating sources of vector development.

The District operates, maintains and services the County-owned streetlights located in areas of public right-of-way in a number of different unincorporated communities of the County. Maintenance of the streetlights includes repair, removal or replacement of all, or part of, any streetlight. The District reimburses Pacific Gas & Electric (PG&E) for services rendered to PG&E-owned streetlights, including the cost of electricity for all lights within the District. The County Roads and Airports Department provides the maintenance and program management services on behalf of the District. The District acts as a financing mechanism to reimburse the County and PG&E for these services, and does not maintain its own dedicated staff.

The District owns, maintains and operates a recreation facility and offers the following recreation programs at the facility: swimming pool activities, Kids Night Out, after-school activities, facility and barbeque rentals, a snack bar, and a location for community-related activities.

The District does not operate a hospital but is the sole member of the non-profit El Camino Hospital Corporation, which operates the El Camino Hospital in Mountain View and which comprises three other affiliated entities including Concern Employee Assistance Program, El Camino Hospital Foundation and Silicon Valley Medical Development. The District provides grants and sponsorships through its Community Benefit Plan to fund health services for the underserved.

The District provides wastewater collection services to the CordeValle community. It also provides wastewater treatment services at its treatment plant, roadway maintenance on 2.5 miles of streets, stormwater drainage maintenance, maintenance of the lake system and the related wells, and landscaping of common areas within the community of CordeValle.

The District owns and operates Rancho Cañada del Oro Open Space Preserve, Sierra Vista Open Space Preserve, Coyote Valley Open Space Preserve and Máyyan 'Ooyákma-Coyote Ridge Open Space Preserve. The District also manages and maintains properties that are currently closed to the public, including Diablo Foothills, Palassou Ridge, Coyote Valley Conservation Area, El Toro, and Santa Cruz Mountains properties. The District provides a variety of activities that are free to the public, such as guided nature hikes, bike and equestrian rides, photography walks, educational programs for children, and stargazing sessions. The District engages in resource management, such as protecting waterways and natural resources and removing invasive plants, preserving cultural artifacts and supporting agriculture and ranching. The District has two active grant programs that provide funds for urban subjects, the Urban Grant Program and the 20% Funding Program.

The District provides collection, treatment and disposal of wastewater with a central collection system utilizing on-site septic tanks and small diameter effluent sewers and a community leach field system.

The District owns and manages over 70,000 acres of land in 27 open space preserves, 25 of which are open to the public free of charge, featuring more than 250 miles of trails. The open space preserves are open, 365 days a year, from a half-hour before sunrise until a half-hour after sunset. Open space preserves are generally kept in a natural condition in order to best protect the environment and wildlife habitat and are developed with only the amenities needed to provide low-intensity recreation. In addition to preserve maintenance, the District is involved in resource management, which includes management of both natural and cultural resources. Natural resource management generally consists of protecting, restoring, enhancing, and monitoring native vegetation and wildlife, and monitoring and protecting the quality of geological and hydrological conditions. Cultural resource management consists of identifying and evaluating and protecting archeological sites, key historical structures, and cultural landscapes.

The District is a non-regulatory agency with the mission of advising and assisting individuals and public agencies in the prevention of soil erosion, runoff control, development and use of water, land use planning, conservation of wildlife and other related natural resources. The District accomplishes its mission by promoting public awareness of the continuing need for resource conservation through educational workshops, informational fliers and papers, planning partnerships, and hands on cleanup or restoration projects, and as a conduit for or source of grant financing.